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ATG4D (C-Terminus) antibody

Cysteine protease ATG4D, AUT-like 4 cysteine endopeptidase, Autophagin-4, Autophagy-related cysteine endopeptidase 4, Autophagy-related protein 4 homolog D, Cysteine protease ATG4D, mitochondrial
Gene name: ATG4D, APG4-D, APG4D, AUTL4
Cysteine protease ATG4D: Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy.

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Ref : 00023102

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Product Code pab73953
Immunogen Synthetic peptide CSQLAQPTLRLPRT from an internal region (near C-terminus) of human ATG4D (NP_116274.3). Percent identity with other species by BLAST analysis: Human (100%), Gorilla (100%), Gibbon (93%), Monkey (93%), Marmoset (93%), Panda (93%)
Target Specificity Reacts with the C-terminus part of human and gorilla ATG4D. Predicted to ross reacts with gibbon, monkey, dog and horse protein.
Name ATG4D
Uniprot ID Q86TL0
Gene ID 84971
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Product type Primary antibodies
Clonality Polyclonal antibody
Produced in Goat
Species Human, Monkey
Labelling None
Appearance Liquid
Conc. / Activity 0.5 mg/ml
Form Antigen affinity purified
Constituents TBS, pH 7.3, BSA 0.5%
Preservatives NaN3 0.02%
Storage Store at -20°C. Minimize freezing and thawing.
Applications IHC - Paraffin sections, ELISA
Working dilutions Optimal dilution should be determined by the end user.
The following are guidelines only :
ELISA (1:32000),
IHC-P (2.5 - 3.75 µg/ml)
Research areas Autophagy
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IHC : ATG4D (C-Terminus) antibody<br/>(pab73953)<br/>Anti-ATG4D antibody IHC staining of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody pab73953 concentration 2.5 ug/ml.

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