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Anti-rabbit IgG (H+L) antibody [HRP]

The quality of this antibody is guaranteed because a very stringent purification protocol is followed where non-specific and specific purifications alternate (affinity chromatography). Antibodies undergo a routine inspection of the cross reactions between target species. If necessary, the total elimination of cross-reactions can require an
adsorption by affinity chromatography against the antigen whose recognition is not desired.

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Product Code lab0273
Immunogen Rabbit IgG (H+L)
Target Specificity Reacts with rabbit IgG. Do not cross react with human, mouse or chicken IgG, A, M
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Product type Secondary antibodies
Clonality Polyclonal antibody
Produced in Goat
Species Rabbit
Labelling Horseradish peroxidase (HRP)
Appearance Liquid
Form Antigen affinity purified
Constituents PBS, Glycerol 50%, BSA 1%, (pH 7.4)
Preservatives Thiomersal 0.02%
Recommendations Peroxidase labeling has the advantage of being sensitive and permanent. Peroxidase substrates are varied: precipitating substrates for western blot or immuno-histochemistry (DAB, AEC, 4CN, TMB), soluble substrates for ELISA (TMB, OPD, ABTS) and chemiluminescent substrates (luminol). However, peroxidase labeling has some disadvantages: Peroxidase is rendered inactivated by oxygen, hypochloric acid and sodium azide. It is therefore advisable to use highly purified water. The presence of endogenous peroxidase in certain tissues like macrophage,
red blood cells, bone marrow cells and plants could increase the background.
Storage Short term storage at +4°C.
For extended periods store in aliquots at -20°C.
Antibodies are guaranteed for 6 month from date of receipt.
Name Rabbit IgG (H+L)
Applications Western Blot, Immunohistochemistry, Immunocytochemistry, ELISA
Working dilutions Optimal dilution should be determined by the end user.
The following are guidelines only :
ELISA (1:2000 - 1:10000)
WB (1:2000 - 1:10000)
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Anti-rabbit IgG (H+L) antibody [HRP]

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