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AKT1 (pSer473) antibody

RAC-alpha serine/threonine-protein kinase
Protein kinase B (PKB)
Protein kinase B alpha (PKB alpha)
Proto-oncogene c-Akt
Gene name: AKT1, AKT, Akt-1, Akt/pkb, MGC99656, Pan-AKT, PKB-ALPHA, PKBalpha, PRKBA, RAC, RAC-ALPHA, V-akt murine thymoma viral oncogene homolog 1
AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis.

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Ref : 00015051

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Product Code pab70039
Immunogen Synthetic phospho-peptide from human AKT1 pSer473
Target Specificity Reacts with the C-terminus aa 460-480 (containing pSer473) of human, mouse, rat and chicken AKT proteins
Name AKT1 pSer473
Uniprot ID P31749
Gene ID 207
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Product type Primary antibodies
Clonality Polyclonal antibody
Produced in Rabbit
Species Human
Labelling None
Appearance Liquid
Conc. / Activity 1 mg/ml
Form Antigen affinity purified
Constituents K phosphate 20 mM, NaCl 150 mM, pH 7.2
Preservatives NaN3 0.01%
Storage +4°C or -20°C, Avoid repeated freezing and thawing.
Applications Western Blot, IHC - Paraffin sections, Immunofluorescence, ELISA
Working dilutions Optimal dilution should be determined by the end user.
The following are guidelines only:
ELISA (1/15000 - 1/60000),
IHC - P (5 µg/ml),
WB (1/200 - 1/1000)
Research areas Apoptosis,Cancer,Kinases and phosphatases,Neurology,PI3K | AKT | mTOR
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IHC : AKT1 (pSer473) antibody<br/>(pab70039)<br/>Anti-AKT1 pSer473 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 µg/ml.

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