Hippo Pathway

Hippo-related products from Covalab

 

TAZ and YAP1

Yes-associated protein (YAP1) and Transcriptional coactivator with PDZ-binding motif (TAZ) are the main downstream mediators of the Hippo pathway.

YAP1/TAZ complex plays a crucial role in organ size control and tumour suppression by restricting proliferation and promoting apoptosis. Phosphorylation of YAP1/TAZ complex by LATS1 and 2 kinases blocks its translocation in to the nucleus and thus its transcriptional co-activator function on cellular genes, important for cell death, proliferation and migration.

TAZ Immunofluorescence

Anti-TAZ antibody IF staining of HeLa cells surexpressing TAZ protein.

TAZ Western Blot

Anti-TAZ antibody WB staining of MDCK cells.

TAZ Western Blot

Anti-TAZ antibody WB staining of HEK 293 cell lysate.

TAZ Immunofluorescence

Anti-TAZ antibody ICC/IF staining of HepG2 cells (green). Nuclei (Blue) are counterstained with Hoecsht 33258.

YAP1 Immunohistochemistry

Anti-YAP1 antibody IHC staining of formalin-fixed, paraffin-embedded human breast.

YAP1 Immunofluorescence

Anti-YAP1 antibody IF staining of HeLa cells.

YAP1 Western Blot

Anti-YAP1 antibody WB staining of HeLa cells lysates.

YAP1 Detection limit

Detection limit for recombinant GST tagged YAP1 is approximately 0.03 ng/ml as a capture antibody.

YAP1 Immunofluorescence

Anti-YAP1 antibody IF staining of HeLa cells.

YAP1 Immunohistochemistry

Anti-YAP1 antibody IHC staining of formalin-fixed, paraffin-embedded human uterus.

YAP1 Western Blot

Anti-YAP1 antibody WB staining of HeLa NE cell lysates.

YAP1 Immunofluorescence
YAP1 Immunofluorescence
YAP1 Immunofluorescence

Anti-YAP1 antibody IF stainings of HeLa cells overexpressing YAP1.

YAP1 Western Blot

Anti-YAP1 antibody WB staining of HEK 293 cell overexpressing YAP1.

YAP1 Immunofluorescence

Anti-YAP1 antibody IF staining of HeLa cells (green). Alpha tubulin and nuclei were counterstained with Dylight 550 (red) and DAPI (blue), respectively.

Other core proteins

The core of the Hippo pathway also includes a kinase cascade starting by MST1 and MST2, which phosphorylate and activate LATS1 and LATS2 kinases. LATS1 and 2 phosphorylate the YAP1/TAZ complex, which maintains it within the cytoplasm. Upon dephosphorylated conditions, this complex is translocated into the nucleus and binds DNA in complex with TEAD1 to TEAD4 (TEA domain-containing-sequence-specific transcription factors).

LATS1 Immunofluorescence
LATS1 Immunofluorescence

Anti-Serine/threonine-protein kinase LATS2 antibody IF stainings of HeLa cells transfected with Serine/threonine-protein kinase LATS2.

MST2 Immunofluorescence

Anti-MST-2 antibody IF staining of HeLa cells transfected with MST-2.

TEAD2 Immunofluorescence

Anti-TEAD-2 antibody IF staining of human breast tumors cells treated (+) or not (-) three days of TGFβ.

TEAD2 Western Blot

Anti-TEAD-2 antibody WB staining of human breast tumors cells treated (+) or not (-) three days of TGFβ.

TEAD4 Immunofluorescence

Anti-TEAD-4 antibody IF staining of HeLa cells overexpressing TEAD-4.

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