login/register
questions
contact us
Anti-Swine IgG (H+L), Peroxydase conjugate antibody
has been added to cart
Primary antibody
- ANGIOGENESIS (47)
- APOPTOSIS (68)
- AUTOPHAGY (32)
- BIOMARKERS (70)
- CANCER (323)
- CARDIOVASCULAR (174)
- CELL BIOLOGY (467)
- CHROMATIN (203)
- DEVELOPMENTAL BIOLOGY (44)
- IMMUNOLOGY (84)
- ION CHANNELS (85)
- LIPIDOMIC (66)
- MICROBIOLOGY (18)
- NEUROSCIENCE (394)
- NUCLEAR SIGNALING (202)
- POST TRANSLATIONAL MODIFICATIONS (131)
- PROTEASOME (25)
- SIGNAL TRANSDUCTION (503)
- STEM CELLS (98)
- TOXINS (9)
- VIROLOGY (28)
- TAGS (26)
Secondary antibody
Other products
Anti-Swine IgG (H+L), Peroxydase conjugate antibody
Code: lab0321
Price / Qty:
Inquiry
Inquiry
Availibility: 24 - 48 h depending on stocks availabilities
If you want to order offline:
Covalab FRANCE: enquiries@covalab.com - tel: +33(0)437654230 - fax: +33(0)437289416
Covalab UK: enquiries@covalab.co.uk - tel: +44(0)1223421055 - fax: +44(0)1223420844
Covalab FRANCE: enquiries@covalab.com - tel: +33(0)437654230 - fax: +33(0)437289416
Covalab UK: enquiries@covalab.co.uk - tel: +44(0)1223421055 - fax: +44(0)1223420844
Code
lab0321
Product
Anti-Swine IgG (H+L), Peroxydase conjugate antibody
Description
Rabbit anti-swine IgG (H+L) purify antibody
Clonality
Polyclonal Antibody
Form
Purified antibodies
Raised In
Rabbit
Immunogen
IgG(H+L)
Target
swine
Labelling
HRP
Specificity
Peroxidase labelling has the advantage of being sensitive and permanent.
Peroxidase substrates are varied: precipitating substrates for western blot or immuno-histochemistry (DAB, AEC, 4CN, TMB), soluble substrates for ELISA (TMB, OPD, ABTS) and chemiluminescent substrates (luminol).
However, peroxidase labelling has some disadvantages: Peroxidase is rendered inactivated by oxygen, hypochloric acid and sodium azide.
It is therefore advisable to use highly purified water.
The presence of endogenous peroxidase in certain tissues like macrophage, red blood cells, bone marrow cells and plants could increase the background.
Peroxidase substrates are varied: precipitating substrates for western blot or immuno-histochemistry (DAB, AEC, 4CN, TMB), soluble substrates for ELISA (TMB, OPD, ABTS) and chemiluminescent substrates (luminol).
However, peroxidase labelling has some disadvantages: Peroxidase is rendered inactivated by oxygen, hypochloric acid and sodium azide.
It is therefore advisable to use highly purified water.
The presence of endogenous peroxidase in certain tissues like macrophage, red blood cells, bone marrow cells and plants could increase the background.
Applications
Antibodies are particularly suitable for use in the most sensitive procedures (ELISA, immuno-histochemistry, immuno-cytology and western blot).
Their quality is guaranteed because a very stringent purification protocol is followed where non-specific and specific purifications alternate (affinity chromatography).
Antibodies undergo a routine inspection of the cross reactions between target species.
If necessary, the total elimination of cross-reactions can require an adsorption by affinity chromatography against the antigen whose recognition is not desired.
Their quality is guaranteed because a very stringent purification protocol is followed where non-specific and specific purifications alternate (affinity chromatography).
Antibodies undergo a routine inspection of the cross reactions between target species.
If necessary, the total elimination of cross-reactions can require an adsorption by affinity chromatography against the antigen whose recognition is not desired.
Working dilutions
ELISA : 1/500 to 1/5000
ICC/IHC : 1/20 to 1/100
IB : 1/100 to 1/1000
ICC/IHC : 1/20 to 1/100
IB : 1/100 to 1/1000
Appearance
Liquid
Constituents
TBS, 50% Glycerol
Preservative
None
Storage instructions
Short term storage : +4°C
Long term storage : -20°C
Long term storage : -20°C
Recommendations
Product in glycerol
Do not freeze
Do not distribute the HRP conjugated in plastics (e.g. eppendorf) so as to avoid a loss of activity
Do not freeze
Do not distribute the HRP conjugated in plastics (e.g. eppendorf) so as to avoid a loss of activity
