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Rabbit anti-sheep IgG (H+L) Fab fragment
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Primary antibody
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Secondary antibody
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Rabbit anti-sheep IgG (H+L) Fab fragment
Code: pab0080
Price / Qty:
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Inquiry
Availibility: 24 - 48 h depending on stocks availabilities
If you want to order offline:
Covalab FRANCE: enquiries@covalab.com - tel: +33(0)437654230 - fax: +33(0)437289416
Covalab UK: enquiries@covalab.co.uk - tel: +44(0)1223421055 - fax: +44(0)1223420844
Covalab FRANCE: enquiries@covalab.com - tel: +33(0)437654230 - fax: +33(0)437289416
Covalab UK: enquiries@covalab.co.uk - tel: +44(0)1223421055 - fax: +44(0)1223420844
Code
pab0080
Product
Rabbit anti-sheep IgG (H+L) Fab fragment
Clonality
Polyclonal Antibody
Form
Fab fragments
Raised In
Rabbit
Immunogen
IgG(H+L)
Target
sheep
Labelling
unlabelling
Applications
Unlabelled antibodies are often used to sensitive ELISA plates or during competition titration.
They can be used in immunoprecipitation, where it is better to use unpurified antibodies (globulin fraction).
The Fab is monovalent fragments of immunoglobulins, i.e. including one site for the fixation of the antigen.
They are prepared by enzymatic digestion (papain).
Like our others antibodies, they are purified by affinity chromatography and are thus particularly specific.
Fab fragments have certain advantages with respect to the entire antibodies in flow cytometry, immuno-histochemistry and immuno-cytology.
On the one hand their specificity is increased since they can no longer be bound in a non-specific way by their Fc part as entire Ig's are.
On the other hand their use also increades the sensitivity of the technique.
In fact, their small size enhances their penetration into tissues and cells as well as their availability for the antigens (particularly for Fab).
Finally, conjugated Fab's have a label/antigenic ratio that is twince as high compared to antibodies (except fluorescent conjugates).
They can be used in immunoprecipitation, where it is better to use unpurified antibodies (globulin fraction).
The Fab is monovalent fragments of immunoglobulins, i.e. including one site for the fixation of the antigen.
They are prepared by enzymatic digestion (papain).
Like our others antibodies, they are purified by affinity chromatography and are thus particularly specific.
Fab fragments have certain advantages with respect to the entire antibodies in flow cytometry, immuno-histochemistry and immuno-cytology.
On the one hand their specificity is increased since they can no longer be bound in a non-specific way by their Fc part as entire Ig's are.
On the other hand their use also increades the sensitivity of the technique.
In fact, their small size enhances their penetration into tissues and cells as well as their availability for the antigens (particularly for Fab).
Finally, conjugated Fab's have a label/antigenic ratio that is twince as high compared to antibodies (except fluorescent conjugates).
Concentration activity
2 mg / ml
Appearance
Liquid
Constituents
PBS, 50% Glycerol
Preservative
None
Storage instructions
Short term storage : +4°C
Long term storage : -20°C
Long term storage : -20°C
Recommendations
Product in glycerol
Do not freeze
Do not freeze
