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Anti-Sheep IgG (H+L), FITC conjugate antibody
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Primary antibody
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Secondary antibody
Other products
Anti-Sheep IgG (H+L), FITC conjugate antibody
Code: lab0299
Price / Qty:
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Availibility: 24 - 48 h depending on stocks availabilities
If you want to order offline:
Covalab FRANCE: enquiries@covalab.com - tel: +33(0)437654230 - fax: +33(0)437289416
Covalab UK: enquiries@covalab.co.uk - tel: +44(0)1223421055 - fax: +44(0)1223420844
Covalab FRANCE: enquiries@covalab.com - tel: +33(0)437654230 - fax: +33(0)437289416
Covalab UK: enquiries@covalab.co.uk - tel: +44(0)1223421055 - fax: +44(0)1223420844
Code
lab0299
Product
Anti-Sheep IgG (H+L), FITC conjugate antibody
Description
Rabbit anti-sheep IgG (H+L) globulin fraction
Other names
Secondary antibody
Clonality
Polyclonal Antibody
Form
Globulin fraction
Raised In
Rabbit
Immunogen
IgG(H+L)
Target
sheep
Labelling
FITC
Specificity
Fluorescein labelling is carried out to obtain a good signal while at the same time minimising the background.
The F/P molar ratio is set between 3 and 7.
FITC has the advantage of being detectible under a microscope.
It can be easily used for carrying out multiple labelling in histochemistry or cyto-chemistry.
However, FITC may rapidly lose its fluorescence.
Maximum absorption: 495 nm.
Maximum emission: 520 nm.
Colour: green yellow.
The F/P molar ratio is set between 3 and 7.
FITC has the advantage of being detectible under a microscope.
It can be easily used for carrying out multiple labelling in histochemistry or cyto-chemistry.
However, FITC may rapidly lose its fluorescence.
Maximum absorption: 495 nm.
Maximum emission: 520 nm.
Colour: green yellow.
Applications
The globulin fraction is obtained after the first level of purification of immune sera. They are prepared by ammonium sulphate precipitation and dialysis.
Globulin fractions show a very good avidity for the antigens.
The elimination of a large part of serum proteins gives a stable product, with reduced background in gel immuno-precipitation techniques.
The use of globulin fractions is not recommended in more sensitive techniques such as enzyme immunoassays.
Globulin fractions show a very good avidity for the antigens.
The elimination of a large part of serum proteins gives a stable product, with reduced background in gel immuno-precipitation techniques.
The use of globulin fractions is not recommended in more sensitive techniques such as enzyme immunoassays.
Working dilutions
IFI : 1/100 to 1/400
Concentration activity
2 mg / ml
Appearance
Liquid
Constituents
PBS, 50% Glycerol
Preservative
None
Storage instructions
Short term storage : +4°C
Long term storage : -20°C
Long term storage : -20°C
Recommendations
Product in glycerol
Do not freeze
Do not freeze
