lab0264

Anti-Rabbit IgG (H+L), Alkaline phosphatase conjugate antibody

Goat anti-rabbit IgG (H+L) Fab fragment

Polyclonal Antibody

Fab fragments

Goat

IgG(H+L)

rabbit

Alk. Phosphatase

Alkaline phosphatase presents a lot of advantages.
It offers a very high sensitivity, it is not rendered inactivated by certain inhibitors like peroxidase is and moreover has got various substrates: precipitating substrates (like BCIP/NTB or NADP/NF), soluble substrates (pNpp) and luminescent substrates (dioetan).
The endogenous activity of this enzyme is particularly strong in the placenta and the intestine.

Do not cross react with human IgG, A, M

The Fab is monovalent fragments of immunoglobulins, i.e. including one site for the fixation of the antigen.
They are prepared by enzymatic digestion (papain).
Like our others antibodies, they are purified by affinity chromatography and are thus particularly specific.
Fab fragments have certain advantages with respect to the entire antibodies in flow cytometry, immuno-histochemistry and immuno-cytology.
On the one hand their specificity is increased since they can no longer be bound in a non-specific way by their Fc part as entire Ig's are.
On the other hand their use also increades the sensitivity of the technique.
In fact, their small size enhances their penetration into tissues and cells as well as their availability for the antigens (particularly for Fab).
Finally, conjugated Fab's have a label/antigenic ratio that is twince as high compared to antibodies (except fluorescent conjugates).

ELISA : 1/500 to 1/5000
ICC/IHC : 1/25 to 1/100
IB : 1/100 to 1/1000

Liquid

TBS, 50% Glycerol, 1% BSA

0.1% NaN3

Short term storage : +4°C
Long term storage : -20°C

Product in glycerol
Do not freeze