Rabbit anti-monkey IgG (H+L) globulin fraction
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Rabbit anti-monkey IgG (H+L) globulin fraction

Code: pab0131
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Code
pab0131
Product
Rabbit anti-monkey IgG (H+L) globulin fraction
Clonality
Polyclonal Antibody
Form
Globulin fraction
Raised In
Rabbit
Immunogen
IgG(H+L)
Target
monkey
Labelling
unlabelling
Applications
Unlabelled antibodies are often used to sensitive ELISA plates or during competition titration.
They can be used in immunoprecipitation, where it is better to use unpurified antibodies (globulin fraction).
Antibodies are particularly suitable for use in the most sensitive procedures : (ELISA, immunohistochemistry, immunocytology and western blot).
The globulin fraction is obtained after the first level of purification of immune sera.
They are prepared by ammonium sulphate precipitation and dialysis.
Globulin fractions show a very good avidity for the antigens.
The elimination of a large part of serum proteins gives a stable product, with reduced background in gel immuno-precipitation techniques.
The use of globulin fractions is not recommended in more sensitive techniques such as enzyme immunoassays.
Working dilutions
Mancini : 1/40 to 1/200
Ouchterlony : 1/5 to 1/10
Optimal dilutions/concentrations should be determined by the end user
Concentration activity
2 mg / ml
Appearance
Liquid
Constituents
PBS, 50% Glycerol
Preservative
None
Storage instructions
Short term storage : +4°C
Long term storage : -20°C
Recommendations
Product in glycerol
Do not freeze