lab0098

Anti-Human GAM Immunoglobulins, FITC conjugate antibody

Goat anti-human IgGAM F(ab')2 fragment

Polyclonal Antibody

F(ab')2 fragments

Goat

IgGAM

human

FITC

Fluorescein labelling is carried out to obtain a good signal while at the same time minimising the background.
The F/P molar ratio is set between 3 and 7.
FITC has the advantage of being detectible under a microscope.
It can be easily used for carrying out multiple labelling in histochemistry or cyto-chemistry.
However, FITC may rapidly lose its fluorescence.
Maximum absorption: 495 nm.
Maximum emission: 520 nm.
Colour: green yellow.

The F(ab')2 is bivalent fragments of immunoglobulins, i.e. including two sites for the fixation of the antigen.
They are prepared by enzymatic digestion (pepsin).
Like our others antibodies, they are purified by affinity chromatography and are thus particularly specific.
F(ab')2 fragments have certain advantages with respect to the entire antibodies in flow cytometry, immuno-histochemistry and immuno-cytology.
On the one hand their specificity is increased since they can no longer be bound in a non-specific way by their Fc part as entire Ig's are.
On the other hand their use also increades the sensitivity of the technique.
In fact, their small size enhances their penetration into tissues and cells as well as their availability for the antigens (particularly for Fab).
Finally, conjugated Fab's have a label/antigenic ratio that is twince as high compared to antibodies (except fluorescent conjugates).

IFI : 1/50 to 1/200
ICC/IHC : 1/10 to 1/100
CMF : 1/10 to 1/100

1 mg / ml

Liquid

PBS, 0.3% BSA

0.1% NaN3

Short term storage : +4°C
Long term storage : -20°C