lab0097

Anti-Human Albumin, Biotin conjugate antibody

Goat anti-human Albumin F(ab')2 fragment

Polyclonal Antibody

F(ab')2 fragments

Goat

Albumin

human

Biotin

The avidin-biotin system is a signal amplification system that allows the detection of quantities of antigens that are too small to be detected by direct enzymatic or fluorescent systems.
Biotin labelled antibodies allow label changes and the use of multiple labels.
Biotin-labelled antibodies have a long storage life.
Moreover, by its small size, the biotin has a negligible effect on the biological activity of proteins.
The presence of endogenous biotin could however, increase background.

The F(ab')2 is bivalent fragments of immunoglobulins, i.e. including two sites for the fixation of the antigen.
They are prepared by enzymatic digestion (pepsin).
Like our others antibodies, they are purified by affinity chromatography and are thus particularly specific.
F(ab')2 fragments have certain advantages with respect to the entire antibodies in flow cytometry, immuno-histochemistry and immuno-cytology.
On the one hand their specificity is increased since they can no longer be bound in a non-specific way by their Fc part as entire Ig's are.
On the other hand their use also increades the sensitivity of the technique.
In fact, their small size enhances their penetration into tissues and cells as well as their availability for the antigens (particularly for Fab).
Finally, conjugated Fab's have a label/antigenic ratio that is twince as high compared to antibodies (except fluorescent conjugates).

ELISA : 1/1000 to 10000
ICC/IHC : 1/50 to 1/200
IB : 1/200 to 1/2000

Liquid

PBS, 50% Glycerol

None

Short term storage : +4°C
Long term storage : -20°C

Product in glycerol
Do not freeze