opr0027

Recombinant human tissue Transglutaminase (His6-rhTG2)

His6-rhTG2 is based on the TGM2-allele from I.M.A.G.E.-clone IMAGp958L121020 isolated from neuroblastoma cells of the human brain.
It is N-ter fused to His6 TAG resulting in the encoded N-ter amino acid sequence MAHHHHHHAEELV...
His6-rhTG2 is produced in E.Coli and purified by ion metal chelating chromatography to more 90% purity.

Recombinant human tissue transglutaminase (rhtTG)

Produced in E.coli

His6-rhTG2 catalyzes acyl transfer reactions from glutamine residues in proteins or peptides to primary amines. It may also be used for immunoprecipitation.

Purity : >90%
Activity: > 750 U/mg
[Activity is determined by measuring the rate of fluorescence enhancement after His6-rhTG2-catalyzed monodansylcadaverine-incorporation into N,N-dimethylated casein according to Lorand et al., Anal. Biochem. 44 (271-231).
1 U is defined as the increase in fluorescence intensity of 1 a.u./min (measured on a Cary eclipse fluorescence spectrophotometer, Varian; lex = 332 nm, lem = 500 nm; band filter = 5 nm; detector strength = 600 V; temperature = 37°C, assay volume = 1 ml)].

White lyophilized solid

The TG is Lyophilised from 50mM NaH2PO4, 150 mM NaCl, pH8 and less than 0.1 mM Imidazole. Sample contains maltodextrin His6_rhTG2 is a Ca2+ dpt enzyme.

Store working aliquots at a minimum of -20°C
Avoid repeated freezing and thawing

Add 150 µl/ 600µl of H20 to the vial of lyophilized powder.
Rotate vial gently until solid dissolves.
After reconstitution the solution should be stored frozen in working aliquots Add 10mM Ca2+ to activate His6-rhTG2.