opr0001

Transglutaminase Colorimetric Microassay Kit

Kits

The Tgase-Covtest uses CBZ-Gln-Gly as the first substrate (amine acceptor) and biotin cadaverine as second (amine donnor).
In the 1st step, samples suspected of containing TGase are incubated with calcium, dithiothreitol (DTT) and biotinylated cadaverine in the wells of microtiter plates to which CBZ-Gln-Gly had been covalently coupled previously.
In the presence of TGase biotinylated cadaverine is incorporated into the g carboxamide of the glutaminyl residue of the dipeptide to form g-glutamyl cadaverine biotin.
In the second step streptavidin-labelled peroxidase is added to the wells.
In the third step, peroxidase activity is revealed using H2O2 as HRP substrate and tetramethyl benzidine as electron acceptor (chromogen)

A colorimetric microassay for quantification of transglutaminase activity

3x96-well plates,
1 ml Negative control,
3 vials Biotin-cadaverine,
50 µl Enzyme tracer,
20 ml Diluent buffer,
50 ml HRP substrate,
1.25 ml Chromogen,
25 ml Blocking reagent

Biotin-cadaverine and Enzyme tracer at -20°C
Other reagents at +4°C