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Specific Plasmatic Transglutaminase (Factor XIII/ FXIII) Colorimetric Microassay Kit : FXIII-CovTest
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Primary antibody
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Secondary antibody
Other products
Specific Plasmatic Transglutaminase (Factor XIII/ FXIII) Colorimetric Microassay Kit : FXIII-CovTest
Code: opr0037
Price / Qty:
350€ / 1Kit
350€ / 1Kit
Availibility: 24 - 48 h depending on stocks availabilities
If you want to order offline:
Covalab FRANCE: enquiries@covalab.com - tel: +33(0)437654230 - fax: +33(0)437289416
Covalab UK: enquiries@covalab.co.uk - tel: +44(0)1223421055 - fax: +44(0)1223420844
Covalab FRANCE: enquiries@covalab.com - tel: +33(0)437654230 - fax: +33(0)437289416
Covalab UK: enquiries@covalab.co.uk - tel: +44(0)1223421055 - fax: +44(0)1223420844
Code
opr0037
Product
Specific Plasmatic Transglutaminase (Factor XIII/ FXIII) Colorimetric Microassay Kit : FXIII-CovTest
Description
The FXIII-Covtest uses biotinylated F11 peptide (Biotin-pepF11) as the first substrate (amine-acceptor/acyl-donor) and spermine as second substrate (amine-donor/acyl-acceptor).
Samples suspected of containing FXIII are incubated with calcium, dithiothreitol (DTT) and Biotin-pepF11 in the wells of microtiter plates to which spermine had been covalently coupled, previously. In the presence of FXIII, spermine is incorporated into the γ carboxamide of the glutaminyl residue of the biotin-pepF11 to form a biotin-pepF11- γ-glutamyl spermine.
The system is coupled to Streptavidin labelled peroxidase (SAv-HRP). SAv-HRP is revealed using H2O2 as HRP substrate and tetramethyl benzidine as electron acceptor (chromogen).
Samples suspected of containing FXIII are incubated with calcium, dithiothreitol (DTT) and Biotin-pepF11 in the wells of microtiter plates to which spermine had been covalently coupled, previously. In the presence of FXIII, spermine is incorporated into the γ carboxamide of the glutaminyl residue of the biotin-pepF11 to form a biotin-pepF11- γ-glutamyl spermine.
The system is coupled to Streptavidin labelled peroxidase (SAv-HRP). SAv-HRP is revealed using H2O2 as HRP substrate and tetramethyl benzidine as electron acceptor (chromogen).
Other names
Specific Plasmatic Transglutaminase (Factor XIII/ FXIII) Colorimetric Microassay Kit
Applications
A colorimetric microassay for quantification of Factor XIII activity
Constituents
Microtiter strips with covalently bound spermine
DTT
EDTA (Negative Control)
Reaction Buffer (Biotin-pepF11/CaCl2)
Enzyme Tracer (SAv-HRP)
Wash Buffer 10X
Diluent Buffer 10X
HRP Substrate
Blocking Reagent
DTT
EDTA (Negative Control)
Reaction Buffer (Biotin-pepF11/CaCl2)
Enzyme Tracer (SAv-HRP)
Wash Buffer 10X
Diluent Buffer 10X
HRP Substrate
Blocking Reagent
Storage instructions
DTT and SAv-HRP enzyme at -20°C.
Other reagents at +4°C.
Other reagents at +4°C.
