We can provide a wide range of modifications such as:

• Phosphorylation
• Methylation
• Nitratation
• Acetylation
• Glycosylation
• ...

Do not hesitate to contact us for other modifications.

2 peptides will be synthesized to perform a project:

• 1 modified peptide: <16 AA, 20-25 mg at >70% of purity
• 1 non-modified peptide: <16 AA, 5-10 mg at >70% of purity

Nevertheless, you can choose to synthesize peptides with other quantities, purities, sequence length or adding modifications.

Normally, peptide is conjugated to a carrier protein via a cysteine residue. If the amino acid sequence of the peptide does not contain a natural cysteine, we add a cysteine to Nter or Cter sequence of the peptide.

• Only the modified peptide is conjugated to a carrier protein
• Carrier proteins available: Keyhole Limpet Hemocyanin (KLH), Bovine Serum Albumin (BSA) or Ovalbumin (OVA)

Two rabbits will be immunized with the modified peptide following our 88 Days protocol.
According to the results, protocol may be changed after discussion with customer.

Each test bleeds will be tested against each peptide by ELISA.
These ELISA test will allow you to follow the evolution of the immuno-reactivity of the rabbits during the immunization process.

Covalab performed a specific immune-affinity purification that allows obtaining specific antibody against site specific modification of protein.

• 2 Immuno-Affinity columns will be prepared (1 with the modified and 1 with the non-modified peptide)
• Purification in two steps:
  • Loading of 5mL of sera on the non-modified peptide agarose column
  • Loading of the non-retain sera on modified peptide agarose column

• Immunoassay Development
• Others Antibody Services